2CXX
Crystal structure of a probable GTP-binding protein engB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-25 |
| Wavelength(s) | 0.979117 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.357, 75.357, 79.112 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.060 - 1.700 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 55008 | |
| <I/σ(I)> | 23.9457 | 3.52895 |
| Completeness [%] | 99.4 | 95 |
| Redundancy | 7.3918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | PEG4000, magnesium sulfate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
