2CXL
RUN domain of Rap2 interacting protein x, crystallized in I422 space group
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-16 |
| Wavelength(s) | 0.97888, 0.97951, 0.97600, 0.98300 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 112.774, 112.774, 76.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.100 - 3.200 |
| R-factor | 0.28 |
| Rwork | 0.280 |
| R-free | 0.36000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WUS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.290 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Number of reflections | 4293 | |
| <I/σ(I)> | 15.2776 | 5.51505 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.0547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.6 | 293 | PEG4000, Lithium Sulfate, Tris-HCl, pH 7.6, LIQUID DIFFUSION, temperature 293K |
