2CXK
Crystal structure of the TIG domain of human calmodulin-binding transcription activator 1 (CAMTA1)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-12-11 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 0.979092, 0.979445, 0.96400 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 88.882, 88.882, 107.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.010 - 1.850 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.069 | 0.323 |
| Number of reflections | 36316 | |
| Completeness [%] | 96.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | Ammonium sulfate, MES buffer, Cesium chloride, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
