2CXI
Crystal Structure Of An N-terminal Fragment Of The Phenylalanyl-tRNA Synthetase Beta-Subunit From Pyrococcus Horikoshii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97928, 0.97960, 0.98400 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.779, 81.973, 139.096 |
Unit cell angles | 90.00, 116.79, 90.00 |
Refinement procedure
Resolution | 41.520 - 1.940 |
R-factor | 0.18981 |
Rwork | 0.188 |
R-free | 0.23070 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.546 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Number of reflections | 104810 | |
Completeness [%] | 99.4 | 94.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch, modified | 5.6 | 293 | PEG 20000, sodium citrate, pH 5.6, Microbatch, modified, temperature 293.0K |