2CXF
RUN domain of Rap2 interacting protein x, crystallized in C2 space group
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-03 |
| Wavelength(s) | 0.97899, 0.97935, 0.96400 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.619, 41.592, 62.132 |
| Unit cell angles | 90.00, 105.95, 90.00 |
Refinement procedure
| Resolution | 14.990 - 3.070 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.29100 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 3507 | |
| <I/σ(I)> | 9.07169 | 6.35079 |
| Completeness [%] | 88.4 | 63.2 |
| Redundancy | 3.4069 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 300 | PEG20000, Bicine, Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
