2CX9
Crystal structure of acyl-CoA dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-30 |
Detector | RIGAKU |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.685, 88.637, 119.557 |
Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
Resolution | 49.550 - 2.000 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 104174 | |
<I/σ(I)> | 16.1 | |
Completeness [%] | 97.7 | 85.4 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 16.5% PEG20000, 0.1M HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |