2CX8

Crystal structure of methyltransferase with ligand(SAH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-02-18
Detector	RIGAKU
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	53.866, 77.821, 55.085
Unit cell angles	90.00, 115.12, 90.00

Refinement procedure

Resolution	48.770 - 2.530
R-factor	0.223
Rwork	0.223
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.620
High resolution limit [Å]	2.530	2.530
Number of reflections	13895
<I/σ(I)>	44.7
Completeness [%]	99.2	94.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG3350, 0.2M potassium formate, 1mM SAH, VAPOR DIFFUSION, HANGING DROP, temperature 293K