2CX7

Crystal structure of sterol carrier protein 2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-06-18
Detector	RIGAKU
Wavelength(s)	0.9790, 0.9793, 0.964
Spacegroup name	P 21 21 21
Unit cell lengths	50.485, 64.512, 70.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.080 - 1.750
R-factor	0.213
Rwork	0.213
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	23346
<I/σ(I)>	22.8
Completeness [%]	97.8	95
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% PEG4000, 0.2M Ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K