2CX4
Crystal structure of a bacterioferritin comigratory protein peroxiredoxin from the Aeropyrum pernix K1 (form-2 crystal)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-06 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.96400, 0.97884, 0.97946 |
Spacegroup name | P 41 |
Unit cell lengths | 132.479, 132.479, 106.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.320 - 2.300 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.26300 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 143980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 285 | ammonium sulfate, MES, Tris, sodium chloride, dithiothreitol, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 285K |