2CX1
Crystal structure of a PUA domain (APE0525) from the Aeropyrum pernix K1 (tartrate complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-17 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.271, 49.139, 53.491 |
| Unit cell angles | 90.00, 103.21, 90.00 |
Refinement procedure
| Resolution | 35.740 - 1.800 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 17683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, Sodium tartrate, Sodium chloride, Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
