2CX0
Crystal structure of a PUA domain (APE0525) from the Aeropyrum pernix K1 (sulfate complex)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-15 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.96300, 0.97916, 0.97948 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.331, 48.135, 53.847 |
| Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
| Resolution | 30.020 - 1.800 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.19900 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 33103 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | ammonium sulfate, Tris, PEG3350, sodium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
