2CWT
Catalytic base deletion in copper amine oxidase from arthrobacter globiformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 157.668, 63.840, 184.453 |
| Unit cell angles | 90.00, 112.40, 90.00 |
Refinement procedure
| Resolution | 30.015 - 1.820 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.950 |
| High resolution limit [Å] | 1.800 | 1.850 |
| Rmerge | 0.058 | |
| Number of reflections | 144511 | |
| Completeness [%] | 99.8 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | 1.05M POTASSIUM TARTRATE, 25mM HEPES, pH 6.8, MICRODIALYSIS, temperature 289K |
