2CWS
Crystal structure at 1.0 A of alginate lyase A1-II', a member of polysaccharide lyase family-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-08 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.575, 68.473, 80.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.000 |
| Rwork | 0.103 |
| R-free | 0.13400 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.053 | |
| Number of reflections | 99115 | |
| <I/σ(I)> | 9.5 | 5.89 |
| Completeness [%] | 96.0 | 76 |
| Redundancy | 9.29 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | PEG8000, ammonium sulfate, sodium acetate, pH 4.5, VAPOR DIFFUSION, temperature 293K |
