2CWH
Crystal Structure of delta1-piperideine-2-carboxylate reductase from Pseudomonas syringae complexed with NADPH and pyrrole-2-carboxylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-12 |
Detector | RIGAKU JUPITER |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.972, 68.874, 179.333 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.570 - 1.700 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.19400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.582 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 91945 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Na/K Tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |