2CWG

CRYSTALLOGRAPHIC REFINEMENT AND STRUCTURE ANALYSIS OF THE COMPLEX OF WHEAT GERM AGGLUTININ WITH A BIVALENT SIALOGLYCOPEPTIDE FROM GLYCOPHORIN A

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	111.000, 50.400, 63.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.171
Rwork	0.171
RMSD bond length	0.018
RMSD bond angle	0.044 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	WGA1	20 (mg/ml)
2	1	drop		0.05 (M)
3	1	drop	T5	2000 (daltons)
4	1	drop	ammonium acetate	0.05 (M)
5	1	drop	ethanol	10-15 (%(v/v))
6	1	reservoir	ethanol	40-50 (%)