2CWE

Crystal structure of hypothetical transcriptional regulator protein, PH1932 from Pyrococcus horikoshii OT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-22
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	90.658, 90.658, 131.871
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.630 - 2.700
R-factor	0.211
Rwork	0.211
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uly
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	6118
<I/σ(I)>	25.2	8.42
Completeness [%]	100.0	100
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Oil batch	9	293	0.1M CHES-HCl, 0.75M NaAcetate, pH 9.0, Oil batch, temperature 293K