2CW9
Crystal structure of human Tim44 C-terminal domain
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 108.119, 108.119, 114.056 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.930 - 1.900 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.22100 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 31350 | |
<I/σ(I)> | 25.6 | 3.9 |
Completeness [%] | 99.1 | 95.7 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG400, HEPES, ammonium sulfate, glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |