2CW7
Crystal structure of intein homing endonuclease II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.700, 149.060, 146.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.390 - 2.700 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SEMET PI-TkoII |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.046 | 0.253 |
Number of reflections | 30434 | |
<I/σ(I)> | 13.4 | 1.7 |
Completeness [%] | 93.2 | 79.5 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Ammonium sulfate, Sodium phosphate, Sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |