2CW5
Crystal structure of a conserved hypothetical protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-11 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.90000, 0.97906, 0.97941 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.790, 78.120, 120.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.400 - 1.940 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.062 | 0.313 |
| <I/σ(I)> | 14.2 | 5.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.12 | 7.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.4M Mg sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
