2CVS
Structures of Yeast Ribonucleotide Reductase I
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418, 0.91900, 0.91940 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.511, 117.166, 63.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.21071 |
| Rwork | 0.204 |
| R-free | 0.26693 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.470 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.072 | 0.445 |
| Number of reflections | 23818 | |
| <I/σ(I)> | 20.1 | 2.4 |
| Completeness [%] | 97.6 | 98.9 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 298 | PEG 3350, sodium acetate, ammonium sulfate, pH 6.5, EVAPORATION, temperature 298K |
