2CVQ
Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.290, 85.160, 118.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.060 - 2.080 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Malate Dehydrogenase |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.070 | 2.210 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 43032 | |
Completeness [%] | 90.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |