2CVP
Crystal structure of mouse AMF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.968, 115.883, 73.267 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.18254 |
| Rwork | 0.181 |
| R-free | 0.21852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.053 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 103572 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Cacodylate, PEG8000, NaAcetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
