2CVJ
Crystal Structure of thioredoxin reductase-related protein TTHA0370 from Thermus thermophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-09 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97909, 0.9, 0.97933 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.050, 79.690, 83.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.380 - 2.000 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.970 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.047 | 0.129 |
Number of reflections | 23729 | |
<I/σ(I)> | 21.2 | 10.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.94 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.3M NaH2 Phosphate, 0.9M K2H Phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |