2CVF

Crystal structure of the RadB recombinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	290
Detector technology	CCD
Collection date	2002-05-11
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.014, 82.468, 111.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.600
Rwork	0.235
R-free	0.30400
Structure solution method	MIR
Data reduction software	SAINT
Data scaling software	SAINTPLUS
Phasing software	SHARP
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.700	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	16828
Completeness [%]	99.5	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.2M ammonium sulphate, 30% polyethylene glycol monomethyl ether 2000, 0.1M acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K