2CV8

Crystal structure of tRNA-intron endonuclease from Sulfolobus tokodaii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-27
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9792, 0.9795, 0.9690
Spacegroup name	P 43 21 2
Unit cell lengths	54.308, 54.308, 242.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.390 - 2.800
Rwork	0.226
R-free	0.29900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.570	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	9765
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG4000, iso-propanol, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG4000, iso-propanol, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K