2CV4
Crystal Structure of an Archaeal Peroxiredoxin from the Aerobic Hyperthermophilic Crenarchaeon Aeropyrum pernix K1
Replaces: 1VGSExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-03-01 |
Detector | RIGAKU |
Wavelength(s) | 0.97933, 0.97000, 0.97905 |
Spacegroup name | P 1 |
Unit cell lengths | 76.738, 103.434, 102.814 |
Unit cell angles | 105.18, 92.91, 105.39 |
Refinement procedure
Resolution | 49.200 - 2.300 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.23000 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 2.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 120086 | |
Completeness [%] | 84.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | oil microbatch method | 8 | 291 | ammonium sulfate, MES, dioxane, sodium chloride, DTT, Tris, pH 8, oil microbatch method, temperature 291K |