2CUT
CUTINASE, A LIPOLYTIC ENZYME WITH A PREFORMED OXYANION HOLE
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.300, 61.100, 72.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.181 |
Rwork | 0.181 |
RMSD bond length | 0.012 |
RMSD bond angle | 2.900 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.900 * |
Rmerge | 0.062 * |
Total number of observations | 50967 * |
Number of reflections | 12970 * |
Completeness [%] | 87.6 * |
Redundancy | 3.92 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | cutinase-E600 | 15 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 15-20 (%) | |
3 | 1 | reservoir | MES | 0.1 (M) |