2CUT

CUTINASE, A LIPOLYTIC ENZYME WITH A PREFORMED OXYANION HOLE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	40.300, 61.100, 72.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.181
Rwork	0.181
RMSD bond length	0.012
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.062 *
Total number of observations	50967 *
Number of reflections	12970 *
Completeness [%]	87.6 *
Redundancy	3.92 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cutinase-E600	15 (mg/ml)
2	1	reservoir	PEG6000	15-20 (%)
3	1	reservoir	MES	0.1 (M)