2CUL

Crystal structure of the GidA-related protein from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	90
Detector technology	CCD
Collection date	2002-11-01
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	78.510, 78.510, 66.095
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.720 - 1.650
R-factor	0.18
Rwork	0.180
R-free	0.19900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	28463
Completeness [%]	99.1	91.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	PEG 4000, magnesium chloride, Tris-HCl, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	PEG 4000, magnesium chloride, Tris-HCl, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	PEG 4000, magnesium chloride, Tris-HCl, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K