2CUL
Crystal structure of the GidA-related protein from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2002-11-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 78.510, 78.510, 66.095 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.720 - 1.650 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.19900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 28463 | |
Completeness [%] | 99.1 | 91.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | PEG 4000, magnesium chloride, Tris-HCl, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | PEG 4000, magnesium chloride, Tris-HCl, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | PEG 4000, magnesium chloride, Tris-HCl, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |