2CU9
Crystal structure of Histone chaperone cia1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-09-24 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.623, 41.297, 67.211 |
Unit cell angles | 90.00, 115.65, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.19387 |
Rwork | 0.191 |
R-free | 0.23891 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wg3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.082 | 0.175 |
Number of reflections | 17836 | |
Completeness [%] | 97.7 | 87.4 |
Redundancy | 3.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | PEG 6000, Ammonium Sulfate, pH 8.0, VAPOR DIFFUSION, temperature 293K |