2CU9
Crystal structure of Histone chaperone cia1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-09-24 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.623, 41.297, 67.211 |
| Unit cell angles | 90.00, 115.65, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19387 |
| Rwork | 0.191 |
| R-free | 0.23891 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wg3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.082 | 0.175 |
| Number of reflections | 17836 | |
| Completeness [%] | 97.7 | 87.4 |
| Redundancy | 3.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | PEG 6000, Ammonium Sulfate, pH 8.0, VAPOR DIFFUSION, temperature 293K |
