2CTS

CRYSTALLOGRAPHIC REFINEMENT AND ATOMIC MODELS OF TWO DIFFERENT FORMS OF CITRATE SYNTHASE AT 2.7 AND 1.7 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	104.140, 78.250, 58.400
Unit cell angles	90.00, 78.50, 90.00

Refinement procedure

Resolution	5.000 - 2.000
R-factor	0.161 *
Rwork	0.161
RMSD bond length	0.014
RMSD bond angle	2.600
Refinement software	EREF

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.0 (mg/ml)
2	1	drop	citrate	0.8 (M)
3	1	reservoir	sodium citrate	0.9-1.0 (M)