Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1993-11 |
Detector | MARRESEARCH |
Spacegroup name | P 61 |
Unit cell lengths | 77.300, 77.300, 77.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 1.670 |
R-factor | 0.153 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cdv |
RMSD bond length | 0.030 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((ROTAVATA)) |
Phasing software | SHELXL-93 |
Refinement software | SHELXL-93 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.300 | 1.710 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.079 | 0.265 |
Number of reflections | 28861 | |
<I/σ(I)> | 8.1 | 2.8 |
Completeness [%] | 94.3 | 89.6 |
Redundancy | 8.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | PROTEIN WAS CRYSTALLIZED FROM 75% (V/V) ETHANOL AND 0.05 M SODIUM ACETATE (PH 5.5) |