2CT9
The crystal structure of calcineurin B homologous proein 1 (CHP1)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.34045, 1.34095, 1.32312, 1.37000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.550, 38.530, 89.950 |
| Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.21855 |
| Rwork | 0.216 |
| R-free | 0.26753 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.369 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.061 | 0.221 |
| Number of reflections | 19708 | |
| <I/σ(I)> | 15.3 | 6.3 |
| Completeness [%] | 98.9 | 97.3 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | PEG3350, Lithium Chloride, Calcium Chloride, CAPSO, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
