2CT8

Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met) and methionyl-adenylate anologue

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793, 0.9796, 0.9820
Spacegroup name	P 21 21 21
Unit cell lengths	91.488, 110.614, 161.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.830 - 2.700
R-factor	0.219
Rwork	0.217
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	42827
Completeness [%]	93.1	79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	H2O
2	1	2	H2O