2CT8
Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met) and methionyl-adenylate anologue
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793, 0.9796, 0.9820 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.488, 110.614, 161.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.830 - 2.700 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 42827 | |
Completeness [%] | 93.1 | 79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | H2O | ||
2 | 1 | 2 | H2O |