2CSD
Crystal structure of Topoisomerase V (61 kDa fragment)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.500, 86.400, 175.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.800 - 2.900 |
| R-factor | 0.23973 |
| Rwork | 0.236 |
| R-free | 0.30787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.111 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 25958 | |
| <I/σ(I)> | 6.7 | 2.3 |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | sodium acetate, sodium formate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
