2CSC

Structure of ternary complexes of CITRATE SYNTHASE WITH D-AND L-MALATE: mechanistic implications

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	104.000, 78.100, 58.300
Unit cell angles	90.00, 78.90, 90.00

Refinement procedure

Resolution	6.000 - 1.900 *
R-factor	0.189
RMSD bond length	0.013
RMSD bond angle	2.600
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.098 *
Total number of observations	52144 *
Number of reflections	25075 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *		referred to 3cts *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.0 (mg/ml)
2	1	drop	sodium citrate	0.9 (%)
3	1	drop	CoA	1 (mM)
4	1	reservoir	sodium citrate	1.2 (M)