2CSB
Crystal structure of Topoisomerase V from Methanopyrus kandleri (61 kDa fragment)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.690, 89.770, 189.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.440 - 2.300 |
R-factor | 0.22046 |
Rwork | 0.217 |
R-free | 0.29227 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.592 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.700 | |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 53175 | |
<I/σ(I)> | 9.6 | 2.9 |
Completeness [%] | 98.1 | 94.8 |
Redundancy | 6.7 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | PEG 3350, Na-cacodylate, MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |