2CS7
1.2 A Crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 62.182, 35.895, 72.541 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 72.550 - 1.200 |
R-factor | 0.11341 |
Rwork | 0.112 |
R-free | 0.13498 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.930 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 72.500 |
High resolution limit [Å] | 1.200 |
Number of reflections | 49793 |
Completeness [%] | 93.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 1.6M Ammonium Sulphate, 100mM Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |