2CS7
1.2 A Crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.182, 35.895, 72.541 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.200 |
| R-factor | 0.11341 |
| Rwork | 0.112 |
| R-free | 0.13498 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.930 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.500 |
| High resolution limit [Å] | 1.200 |
| Number of reflections | 49793 |
| Completeness [%] | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 1.6M Ammonium Sulphate, 100mM Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
