2CQZ
Crystal Structure of PH0347 protein from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-07 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.511, 101.166, 78.646 |
Unit cell angles | 90.00, 119.78, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.600 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.22531 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xx7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.542 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.27) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.057 | |
Number of reflections | 32607 | |
<I/σ(I)> | 9 | |
Completeness [%] | 99.7 | 98.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 9 | 295 | Sodium chloride, Bicine, PEG-MME 550, pH 9.0, microbatch, temperature 295K |