2CQT

Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000000
Spacegroup name	P 1 21 1
Unit cell lengths	85.275, 98.800, 105.102
Unit cell angles	90.00, 102.53, 90.00

Refinement procedure

Resolution	41.860 - 2.100
R-factor	0.18
Rwork	0.180
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v7w
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.094	0.303
Number of reflections	99298
<I/σ(I)>	8.1	3.29
Completeness [%]	100.0	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	277	0.8M Sodium/Potassium Phosphate, 10mM glucose, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K