2CQS
Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.840, 98.420, 104.040 |
Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
Resolution | 49.210 - 2.000 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v7w |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.068 | 0.307 |
Number of reflections | 111886 | |
<I/σ(I)> | 8 | 2.82 |
Completeness [%] | 97.0 | 92.1 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.5M Ammonium Sulfate, 0.1M MES-NaOH, 5mM glucose, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |