2CPG
TRANSCRIPTIONAL REPRESSOR COPG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.190, 102.490, 40.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.600 |
R-factor | 0.2017 |
R-free | 0.27720 |
Structure solution method | MIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 0.025 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CCP4 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.610 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.080 | 0.126 |
Total number of observations | 91693 * | |
Number of reflections | 18358 * | |
<I/σ(I)> | 10.3 | 3 |
Completeness [%] | 98.1 | 96.4 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * | Gomis-Rueth, F.X., (1998) FEBS Lett., 425, 161. * |
1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * | Gomis-Rueth, F.X., (1998) FEBS Lett., 425, 161. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | BENZAMIDINE | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13-16 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | reservoir | 275 (mM) | ||
4 | 1 | reservoir | 0.5 (mM) |