2COQ
Structure of new antigen receptor variable domain from sharks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 38.268, 68.324, 39.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.200 - 2.100 |
| R-factor | 0.22042 |
| Rwork | 0.218 |
| R-free | 0.28048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ver |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.126 | 0.977 |
| Number of reflections | 6336 | |
| <I/σ(I)> | 15.2 | 2 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 0.1M Chess PH9.5, 50% PEG200, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
