2CO5

F93 FROM STIV, a winged-helix DNA-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-17
Detector	ADSC QUANTUM 315
Spacegroup name	C 2 2 21
Unit cell lengths	42.050, 102.594, 92.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.370 - 2.200
R-factor	0.192
Rwork	0.190
R-free	0.23200
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	0.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.340
Number of reflections	10466
<I/σ(I)>	33	4.2
Completeness [%]	99.8	100
Redundancy	7.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.75		HANGING DROP VAPOR DIFFUSION, 9.0-10.5 MG/ML F93 IN 10 MM TRIS-HCL, PH 8.0, 50 MM NACL, 0.5 MM TCEP MIXED WITH BUFFER CONTAINING 0.1 M SODIUM ACETATE, PH 4.75-5.0, 0.1 M MAGNESIUM NITRATE HEXAHYDRATE, 16-20% PEG 20,000.