2CNT
RimI - Ribosomal S18 N-alpha-protein acetyltransferase in complex with CoenzymeA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200B |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH RAXIS IV |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 126.926, 126.926, 113.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 113.960 - 2.400 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | SOLVE (RESOLVE ARP WARP AMORE CCP4) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.350 |
| Number of reflections | 39657 | |
| <I/σ(I)> | 24.8 | 4.4 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 2.2-2.8 M (NH4)2SO4, 100 MM MES PH 4.5-5.5 |
