2CNM
RimI - Ribosomal S18 N-alpha-protein acetyltransferase in complex with a bisubstrate inhibitor (Cterm-Arg-Arg-Phe-Tyr-Arg-Ala-N-alpha- acetyl-S-CoA).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200B |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH RAXIS IV |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 133.127, 133.127, 190.986 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.530 - 2.600 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.25700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.602 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 19233 | |
| <I/σ(I)> | 19.4 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 15% PEG400 (W/V), 100 MM NACITRATE PH 5.5, 100 MM (NH4)2SO4 |
