2CNC
Family 10 xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.904, 67.365, 105.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.700 - 2.400 |
| R-factor | 0.161 |
| Rwork | 0.155 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ur1 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.157 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.490 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 11465 | |
| <I/σ(I)> | 14.2 | 4.1 |
| Completeness [%] | 86.0 | 84 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.35 M MGCL2, 0.1 M TRIS, PH 6.5, 30% PEG 4000, 5% PEG 400 |
