2CN7
Recombinant human H ferritin, K86Q, E27D and E107D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-18 |
Detector | MARRESEARCH |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 181.207, 181.207, 181.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 104.830 - 1.750 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2clu |
RMSD bond length | 0.015 |
RMSD bond angle | 1.296 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.300 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.070 | 0.560 |
Number of reflections | 26251 | |
<I/σ(I)> | 26.5 | 5.34 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.7 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP. RESERVOIR: MPD 5.2%, CACL2 3.9 MM, TRIS 50MM PH 8.5. DROP: 1UL PROTEIN (24 MG/ML) AND 1 UL RESERVOIR |