2CN6

Recombinant human H ferritin, K86Q and E107D mutant, soaked with Zn ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-18
Detector	MARRESEARCH
Spacegroup name	F 4 3 2
Unit cell lengths	178.453, 178.453, 178.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.140 - 2.200
R-factor	0.196
Rwork	0.195
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2CHI
RMSD bond length	0.017
RMSD bond angle	1.413
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.610	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.550
Number of reflections	12895
<I/σ(I)>	28	7
Completeness [%]	99.8	100
Redundancy	23	23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5		HANGING DROP. RESERVOIR: MPD 5% CACL2 4.7 MM, TRIS 50 MM PH 8.5 DROP: 1 UL PROTEIN (22 MG/ML) AND 1 UL RESERVOIR