2CN3
Crystal Structures of Clostridium thermocellum Xyloglucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.440, 141.440, 193.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.950 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cn2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.375 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.070 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.150 | 0.530 |
| Number of reflections | 142508 | |
| <I/σ(I)> | 14.3 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350, HEPES PH 7.5, 0.2M KSCN |
