2CN1
Crystal structure of Human Cytosolic 5'-Nucleotidase III (NT5C3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.389, 100.951, 76.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.650 - 2.670 |
| R-factor | 0.196 |
| Rwork | 0.192 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bdu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.610 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 9828 | |
| <I/σ(I)> | 15.2 | 4.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 24% PEG1500, 20% GLYCEROL, pH 7.50 |
